# Multiple nesting criteria

To nest over multiple values of the same variable

▪For variables like In zone, specify those values in one Nest box.

▪For variables with more than 2 states, like Body elongation state, Activity state and Mobility state, create one Nest box per value, and combine the resulting boxes with OR logic.

example 1 With the variable In zone

You want to visualize the track segments when the subject was entirely in one of the open arms of the Elevated plus maze. Each open arm has been defined as a zone.

1.Create a Nest box corresponding to the variable; in this example, In zone.

2.Specify your selection.

3.Insert the Nest box in the sequence.

Analysis is done on the samples collected when the center point, nose-point and tail-base of the subject were all in either in open arm 1 or in open arm 2 of the plus maze.

notes

▪If the zones do not overlap, from the In the following zones list select When in any of the selected zones (OR logic).

▪If the zones do overlap, and you want to select the time when the subject was in the intersection of those zones, from the In the following zones list select When in all selected zones (AND logic).

example 2 With the variable Activity state

You want to select the track segments when the subject was either Active or Highly active. It is assumed here that Activity state is defined with 3 possible states: Inactive, Moderately active, Highly active.

1.Create a Nest box for the variable Activity state. Under Calculate nesting for, choose Moderately active.

2.Create a second Nest box for Activity state. Under Calculate nesting for, choose Highly active.

3.Combine the Nest boxes with OR logic. See Boxes in parallel = OR Logic.

To nest over two or more variables

Create one Nest box per criterion.

To refine nesting according to two or more nesting criteria, create the corresponding Nest boxes and connect them between the Start box and the Result box using the logic you require.

example You want to visualize the track segments when the animal was completely in the center of the open field AND its body was stretched. The center of the open field was defined as a zone.

If you want to nest over multiple values of the same variable (for example, nest over Highly mobile AND Mobile of the variable Mobility), create one Nest box per value and connect them using the logic you require (AND, OR or Multiple Results).