What are the supported video file formats for EthoVision XT?

Knowledge Base Search

MyNoldus Knowledge Base Related to EthoVision XT What are the supported video file formats for EthoVision XT?

Supported Video File Formats for EthoVision XT 12–16

EthoVision XT supports playback of the following digital video file formats:

  • MPEG-1No longer supported in EthoVision XT 16.
  • MPEG-2 – A collection of formats; not all are supported.
  • MPEG-4 – A collection of formats; not all are supported.
  • MPEG-4 (mp4v) in MP4 container – Created when recording with analog cameras on the Picolo Alert board.
  • MPEG-4 DivX in AVI container – Format used by Media Recorder and EthoVision with digital cameras.
  • H.264 AVC in MPEG container – Created by Media Recorder 2.5 and earlier with Euresys Picolo H.264 board.
  • H.264 AVC in MP4 container – Created by Media Recorder 4.0 with the Picolo H.264 board.
  • H.264 AVC – A type of MPEG-4 that is supported.
  • DV-AVI – Uncompressed video from FireWire cameras. Not recommended due to large file size.

Important Notes:

  • DV-AVI is a special case of AVI. EthoVision does not generally support AVI-format video files, even if the extension is .avi.
  • MPEG-4 files may have the extensions .mpg, .mpeg, or .avi, which does not guarantee compatibility.
  • Only MPEG-2, MPEG-4, and H.264 files created with Noldus-supplied encoder boards and Media Recorder are guaranteed to work.

Video Decoding Support

  • MPEG-1 / MPEG-2: Decoded using MainConcept Decoder (installed with EthoVision XT)
  • MPEG-4: Decoded using the standard Windows decoder
  • H.264: Requires decoding software installed with EthoVision XT

Video Formats Not Supported

  • DivX – except MPEG-4 DivX from Media Recorder
  • WMV
  • QuickTime (.mov)
  • FLV (Flash / YouTube video)
  • XviD variants of MPEG-4

Testing Other Formats

Some unsupported formats may still function. Check for Missing Samples and Interpolated Samples (in Detection Settings or Acquisition).

  • If either metric is above 1% or shown in red, the video may be dropping frames or have inconsistent timing.

Determining the Format

To check the format of your video:

  1. Right-click the file
  2. Select Properties

Video Properties Dialog

Video Resolution Requirements: Resolution must be at least 640 × 480 and no more than 1920 × 1200.

To check resolution:

  1. Right-click the video file
  2. Select Properties > Details

Video Resolution Details

Converting Videos

If your video format is unsupported, use a converter to change the format to a compatible one.

MP4Converter (Recommended)

In the converter:

  • Set Profile to General Video Format
  • Observer / FaceReader: DIVX, XVID, MPEG-1, or MPEG-2
  • EthoVision / DanioScope: MPEG-1, MPEG-2, or DIVX
  • For MTS files: Convert to DIVX or XVID (MPEG-1 and MPEG-2 often fail)

This tool offers a trial to convert the first 3 minutes of a video for compatibility testing.

Handbrake (Free)

Settings in Handbrake:

  • Summary tab: Format = MP4
  • Video tab: Codec = H.264, Frame Rate = “Same as Source”, enable Constant Framerate